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_ @ @ FD-302a (Rev. 10-6-95) 279R-WF-222936-USAMRIID Coninuatonorsosmer _ [ ,0n ApEil 26, 200% 2 bé b7c bvF Ba spore growth in flasks with Leighton-Doi media and using the hot wat /shaker apparatus typically occurred over a duration. | | recat ied occasional instances where, after Starting the procedure on Friday,L[__] returned on Monday to find the Ba vegetative cells had not exhausted their Leighton-Doi media nutrients and therefore had not under gone sporulation, or were in various stages of sporulation (i.e. a mix of_w tative cells, cells under going sporulation, and spores). also noted on other occasions vegetative cell growth was too rampant. In situations like this, the Ba spores that were formed were of poor quality and "clumpy".[____Jadvised the ratio of Ba vegetative cells to media volume was directly proportional to spore quality: too much Ba vegetative cells within a defined media volume results in poor Ba spore quality. [— Jadvised it was standard procedure to allow the Ba vegetative cells to exhaust their nutritive supply in order to induce sporulation. indicated that research s_ not involve Ba. The spores were never dried. advised to knowledge there was no "dry work" conducted a rather it Tt or OH a infection, and this is advantageous in studying certain aspects of