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,To: Washington @.. From: “Washington ric Re: 279A-WF-222936-USAMRIID, 09/07/2006 supports the research of laboratories by providing expertise in cell culture, protein expression, and protein purification as well as growing cells. The contract describes the relationship between the two entities as, "Temporary housing and maintenance of USAMRIID fermentors in exchange for unfettered use by a contractor plus scheduled runs for USAMRIID."> A copy of a photograph | USAMRIID Horizon newspaper article shows Big Bertha and the two .Bidstat Braun Biotech\fermentors at their current off-base location. stated the fermentor was used fairly infrequently due to its_large size.‘ It has had various operators to include: used C. Botulinum between 1991-1993; material material used 1991-1997; E.Coli, B. Subtilis, and delta-Sterne betwee Cc material used_FV-1 antigen;[7——=ds material used Sterne strain of Ba; CT Material used water. Durin lemployment_individuals recalled that used © exmentor in RoomL___]|for attenuated strains of anthrax; protective antigen (PA), lethal factor (LF), ee and edema factor (EF) studies. and has fermented B. subtilis. described a second fermentor as being Big Bertha's twin. It, d to th It is surmised that after the destruction of the facility at USAMRIID this fermentor was relocated and therefore not in place during the anthrax mailings. who was identified as using Big Bertha and its twin, originally obtained these fermentors from the Salk Institute for use with C. botulinum in direct support of vaccine production with a deadline from the Pentagon in support of Operation Desert Storm. Around that time frame, the fermentors were both located_at_USAMRIID_ in Building> | in Biological Production Lab 1 | a) and[ | now known as rooms | and xespectively.® 75-Liter Fermentor: Roor{ | This fermentor, no MMCN Number, no Stock Number, described only as being glass-lined was housed in[—____] during the 1980's. la. 75-liter fermentor located in Room to grow colonies of attenuated ~ delta-Sterne strain Bacillus anthracis."® Other operators 2 b6 b7c b2 b7F