◆ SpookStack

FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID conimaton ott03020¢ _ [om 04/17/2003 Powe 4 Each cryovial of primary stock in the archive contained approximately 0.5 mL of material. A secondary stock of a given B.a. strain was generated from the corresponding primary stock; however, most of the vials in the archive were never used in this manner. To generate a secondary stock of a particular strain, a loop of approximately 10 microliters (wL) was pulle ock and cultured on either agar or broth media. never worked with the original Ames slants and was not aware of how the Ames original slants were received by USAMRIID. never saw any of the original Ames materials and was not sure if the primary poss ta its archive were derived directly from the original slant. no knowledge of the B.a. Ames stocks generated by BRUC. S and believed that IVINS was gone by the time that arrived _at USAMRIID and thought that he gave his B.a. Ames stocks to lescribed in detail the procedures for the shipping of pathogenic samples. The guidelines differed for the transport of virulent and avirulent organisms. For requests of virulent materials, such as B.a. Ames, regulations were folletied, as defined by the Select Agents Program of the CENTER D, EC D PREVENTION (CDC). To receive Select Agents, the/requester had to be registered with the CDC. Prior to shipment of €he pathogenic materials, transfer paperwork, or EA101 documents, were processed through the appropriate channels.