◆ SpookStack

"BD-302a (Rev. 10-6-95) 279R-WF-222936-USAMRIID Continuation of FD-302 of ,0n.06/2/2004 __,Page__2 extra tube of material in case necessary. The extra tube was autoclaved at the end of the day. The material that remained in the nebulizer was always autoclaved. The aerosol challenges were run for j¢ ten (10) minutes. The entire process.to setup the challenge and b7c complete it_would take approximately twenty (20) to twenty-five (25) minutes. noted that there were always a lot of people present for the aeroso For each anthrax aerosol challenge there were always that IVINS, were involved in conducting the aerosol challenges. challenges. [saa that the hood lines were not decontaminated between days OF aerosol exposures. The all glass imp} was passed out to the PI for At the end of the da EY materia rom the aerosol challenges was autoclaved. — the cleaning process after an aerosol challenge as taking one (1) to two (2) hours. It took ninety (90) minutes to run the autoclave in the hood lines. The material collected in the AGI was_kept for at least one loes not recall post-challenge plates or materia. ing around any of the rooms in Building 1412. tated thatLS™~—COC#XY VII would do all of y¢ the post-challenge work following an aerosol challeng he media for pic the aerosol _work was kept in the refrigerator in room The media 52 included The water_used during the anthrax aerosol challenges was provided by IVINS. a CN iillwas used in the aerosol challenges of toxins, however,