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‘ | LED.3028 (Rev. 10-6-95) 279A-WE-222936 Continuation of FD-302 of es | ,On 12/01/2006 __, Page 2 [__]erieves BRUCE IVINS is responsible for the lyophilizer, since i on IVINS's hand receipt, but does not believe IVINS sroweal Jhon tous} t it. There are instructions osted on the lyophilizer and [a Jetieves IVINS' referred to the instructions. shown printouts of e-mails dated (Jin which equesting IVINS to show[__l]how to use the lyophilizer. was familiar with the e-mails, but was not sure that IVINS showed[ ]how to use the lyophilizer, stating that the. lyophilizer was easy to use (user friendly) and the instructions posted on the lyophilzer were good. The first time[____Jusea the_lyophilizer| had difficulties after the defrost cycle. [pened e door to the cold trap and the water inside (condensate from the lyophilized samples) spilled out. stated that t. acuum Bune oil for the suppnitizer had not been changed since According coL___] the _lyophilizer is rarely used (maybe once every s). the lyophilizer is (writer believes to be ever preschedule € use o the lyophilizer, although, would occasionally put a note on the ophilizer prior to use. IVINS sometimes made comments abou fase of = odised. ip tasty SO, oftentimes would te. IVINS before used t! izer. preparations took the lyop flier Po to complete, wanted to be sure the preparations were completely dry so would add [[_______]to assure complete dryness. The cloth was to cross-contamination agreed that it was theoretically possible to cross-contaminateé samples when drying them together. When the lyophilization was complete the that had sublimated from the samples, would have condensed as ice in the cold trap. Once the ice in the cold trap thawed, the water would be removed.