◆ SpookStack

Desc hle ALL FED INFORMATION CONTAINED HEREIN IS UNCLASSIFIED DATE 12-08-2008 BY 60324 UC BAW/RS/LSC Analysis of Sample Date analyzed - 17 October, 2001 Date of Report - 18 October, 2001 Sample. SPS02.57.03. | received the sample (in ziplock bags) from[________Jon the afternoon of 17 October, 2001. The sample was taken into B-3. Insufficient 2 powder was on the letter, so powdery material was scraped from the envelope bye and put into a small, tared, glass container. The container was reweighed and the net weight of the powdery material was determined to bef tote material was added f sterile water for injection to make a total of (and approximatel of suspension. Ten-fold dilutions were plated out onto TSA, then incubated overnight. Plate counts were made, and it was determined that the original tube contained Since there was[___| [ “‘)of material, this calculates to bi lof powder material. Visual inspection of the suspension of material under phase contrast microscopy found no visible vegetative cells, no visible debris, and very few small clumps. Most of the material appeared to be individual refractile spores. Interpretations and conclusions: If this is a preparation of bacterial spores, it is an extremely pure preparation, and an extremely high concentration. These are not "garage" spores. The nature of the spore preparation suggests very highly that professional manufacturing techniques were used in the production and purification of the spores, as well as in converting the spores into an extremely fine powder. Bruce E. Ivins, Ph.D. USAMRIID Bacteriology Division