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. “ e @ FD-302a (Rev. 10-6-95) 279A-WF-222936 SUB 302 Continuation of FD.302 of Bruce Ivins on 08/13/2003 Page 2 £, —RERRY MIKBSELL, dated July, r991, and labeled be Fos that the MIKESELL sample was derive bee stock culture around 1985. The sample labeled b7C and submitted to the Repository in April, 2002 may be from MIKESELL! ion and not directly fromp ‘stocks of BA. submitted IVINS! samples to the and would be able to provide information_on the origin sample in the Repository. IVINS began work at USAMRIID in the early 1980s. When IVINS arrived, the scientists who worked on BA cultured BA, or "sub- cultured" BA by taking an inoculation loop, scraping the loop across a plate of multiple BA colonies, and then using the scraped loop to inoculate a fresh culture for BA growth. IVINS was surprised at this method of inoculation. Before beginning work at USAMRIID, IVINS sub-cultured bacteria by using an inoculation loop to "pick" a single colony of bacteria, instead of taking multiple bacterial colonies as done at USAMRIID. The single colony method was used by many scientists so that sub-culturing would not lead to genetic diversity in growing cultures of bacteria. The multiple bé ~culturing method used at USAMRIID was recommended by b7c a respected scientist in the BA field. In 1983 and e two plasmids present in BA were discovered, and IVINS ‘everted to the single colony method of subculturing. Further, when IVINS reverted to the single colony method, IVINS also used the original 1981 agar slant of the Ames BA and started a fresh culture of Ames to use in his, IVINS, research. IVINS noted_that MIKESELL moved to Ohio several years ago and no longer workéd at USAMRIID. MIKESELL was considered an alcoholic while MIKESELL worked at USAMRIID, due to MIKESELL drinking a lot -of hard liquor. MIKESELL died last year and IVINS attributed MIKESELLy death to excessive drinking, lack of exercise, and a poor th MIKESELL at USAMRIID were, (phonetic) .