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oe b6 u @ ke @ [ee w FD-302 (Rev. 106.95) ALL INFORMATION CONTAINED HEREIN I5 UNCLASSIFIED -1- DATE 12-16-2008 BY 60324 UC BAW/DK/TH , FEDERAL BUREAU OF INVESTIGATION Date of wanscription _09/10/2004 BRUCE E. IVINS, Pri: ae. telephone number ace of employment, Institute of Intectious Disease Fort Detrick, Maryland. After Ber g advised <= the identities of the interviewing agents and the nature of the interview, IVINS provided the following information: ipal Investigator, date of birth In 1985, IVINS was concerned about the possibility of genetic variants of Bacillus anthracis (Ba) making their way into cultures which were grown by sweeping batches of spores off of a seed stock and plating these spores. IVINS noticed that when he took a full sweep of many spores to inoculate a plate (sweep technique), he would get a more heterogeneous culture. In addition, when he passed a culture that was plated as bé described above, each generation of passage would include more TC aberrant colony morphologies. When IVINS used just one colony to inoculate a plate (single colony technique), the result was a more homogeneous culture with just a few aberrant colony morphologies. Although he was advised by the "old timers" like that he should inoculate plates by taking a sweep of spores from the seed stock, IVINS believed that growing spores in this way was contrary to pure microbiology culture technique. As a result, in 1985, IVINS dipped into the original 1981 Ames slant to begin a new seed stock which he refers to as the "1985" sample. IVINS intended to use this sample as a seed stock so that he would not have to continually dip back into the "1981" original slant. He did not recall the sample name, number, or what was written on the tube containing the "1985" sample. He does not think that any of the "1985" sample remains, however, if it does,’ the FBI has it in their repository. IVINS cannot recall if he used just one spore “from the "1981" sample to create his "1985" seed stock or if he used just a few "normal" spores. IVINS noted that there were originally two "1981" original slants; however, there is now only one "1981" slant. IVINS does not know what happened to the second slant. The "1985" sample was being used as seed stock for intramuscular (IM) challenges, therefore USAMRIID was not growing large batches of spores. Large batches of spores in Investigation on 09/08/2004 a Frederick, Maryland re# 279A-WP-222936-USAMRIID ~ 945° Date diated ~—09/10/2004 ‘This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is tage your agency, it and its contents are not to be distributed outside your agency. (a