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FD-302a (Rev. 10-6-95) 279A-WF-222936-BEI Continuation of FD-302 of BRUCE EDWARDS IVINS ,on 02/13/08 » Page 8 After reading the captions and examining the_photographs IVINS explained that he obtained the photographs from[ and typed the captions on them. Although IVINS was abIé To understand what the diagram and captions explained, he could not remember the specific interview which caused him to create them. Additionally, he would not adopt the statements or explanations as his own beliefs. IVINS eventually explained that[ sd woul make several subcultures, or serial passage the organism when growing them. This caused variants or mutations to appear. IVINS did not use serial passages. Rather, his cultures were all grown from the original slant provided to USAMRIID by the USDA, thereby preventing the creation of variants or mutations. The "BI Culture" depicted in the diagram refers not only to RMR-1029, but also to spores that were grown in the same fashion as RMR-1029. So when IVINS wrote that New Mexico, DRES, Battelle, and Dugway received his culture of Ames, only Battelle and New Mexico actually received RMR-1029. DRES received some frozen material, and Dugway was given spores from IVINS' culture so they could grow larger lots which were ultimately used to create RMR-1029. 5 The spores in the photographs were grown on blood agar plates which makes it easy to identify mutations or variants. IVINS never saw the spores from the mail szoun on blood agar, so he bé could not compare them to either his or spores. bic did make that comparison, however, and told IVINS_what ound. Namely, that the spores from the mail looked lik spores and not IVINS'. The only examination IVINS conducted of the spores from the mail occurred when he grew them on tryptic soy agar (TSA) plates. When growing spores on TSA plates, however, it is not’as easy to identify mutations or variants. When growing the spores from the mail on TSA plates, IVINS saw nothing in them which appeared to be an obvious mutation. All of the above mentioned documents which were shown to IVINS-have been placed in a 1-A envelope and made part of the file.