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TO: Washington Field From: Washington Field Re: 279A-WF-222936-USAMRIID, 07/14/2004 component of the anthrax toxin (unknown strain). No date was listed for this test. Notebodk | Notebook [____] issued January 5, 1981 and entitled "hegionnaires' Disease", contained information on Legionnaire's Disease, fermenter runs with Bacillus anthracis Sterne, work with an anthrax vaccine, and other related information. Entries were made by Bruce Ivins. Page 24, dated February 4, 1981, described a medium preparation of anthrax toxin (unspecified strain), which was a modification of the "Haines, Klein, and Lincoln" preparation from the "Journal of Bacteriology", 89: 74-83. ; "Run #1 - Sterne Supe" was listed on page 28 and dated February 12, 1981. "Run #2", with some changes to that experimental procedure, was listed on page 40 and dated March 5, 1981. Ivins also ran tests attempting to purify the Ba toxin or any of its components. On page 48, dated March 19, 1981, Ivins wrote that given me some anthrax vaccine (adsorbed onto aluminum hydroxide) ." The strain used for the vaccine is unknown, but the Ba was of an "avirulent, nonencapsulated strain". Page 64, on April 16, 1981, lists a protocol involving inoculating Ba Sterne from a 24-hour blood plate. Notebodk [| Notebook [| issued April 29, 1981 and entitled "Rapid Detection of Infectious Diseases", contained information about_R_ medium composition, optimum toxin production conditions, the anthrax strain, comparison of media for growth and toxin production, non-toxicity of spores after heat passaging, and other related information. Entries were made by Bruce Ivins. Page 1, dated May 11, 1981, was entitled a medium for B. anthracis." On the next page, three liters of the medium were made, dispensed into flasks, and incubated. On page 4, Ivins detailed a procedure in which he grew five liters of Ba (strain unknown) to give to —oe«"e" a However, no rats died after being injected with this medium; Ivins speculated that he harvested the Ba too early in the growth phase of the organism. On page 18, one liter of bs b7C