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.FD-302a (Rev. 10-6-95) b7c 27 9R-WF-222936 Continuation of FD-302 of | ,On 12/01/2006 _, Page 2 believes BRUCE IVINS is responsible for the lyophilizer, since it_is on IVINS's hand receipt, but does not believe IVINS showed how to_use it. There are instructions osted on the lyophilizer and [_|believes IVINS' referred to the instructions. shown printouts of e-mails dated in which equesting IVINS to show [__ how to use the lyophilizer. was familiar with the e-mails, but was not sure that IVINS showed[___]how to use the lyophilizer, stating that the. lyophilizer was easy to use (user friendly) and the instructions posted on the lyophilzer were good. The first time[ used the lyophilizer had difficulties after the defrost cycle. [____prened e door to the cold trap and the water inside (condensate from the lyophilized samples) spilled out. stated that t acuum pune oil for the aypphi izes had not been changed since According tol the lyophilizer is rarely used (maybe once every two months). the lyophilizer PsP tnriter believes to be fee Sind ever preschedule @€ use oO the lyophilizer, although, would occasionally put a note on the lyophilizer prior to use. IVINS sometimes made comments sbour{ use of the_lyophilizer, so, oftentimes would te IVINS before [fused t Liizer. preparations took the lyophilizer to complete, wanted to be sure the preparations were completely dry so would add[__—~—“*~CSCSJtO0). aS COMpleetee dryness. The cloth was to eep out dus had no problems with cross-contamination since all of samples were the same. agreed that it was theoretically possible to cross-contaminate samples when drying them together. When the lyophilization was complete the that had sublimated from the samples, would have condensed as ice in the cold trap. Once the ice in the cold trap thawed, the water would be removed.