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To: Washington Field From: Washington m4 Re: 279A-WF-222936-USAMRIID, 05/24/2005 found several areas with presumptive positives for the presence of B.a.*? On the following Monday, April 15, 2002, after thinking over the weekend and becoming increasingly more concerned about possible contamination immediately outside the [] suite, IVINS decided to independently conduct environmental surveys in areas likely, in his mind, to have been contaminated. IVINS acknowledged that he should have requested authority from his superiors to conduct the surveys, but he was afraid that it would have taken weeks before permission was granted due to "red- tape."®° IVINS felt that he had a legitimate concern due to the possibility of the area being contaminated.* ° IVINS' April 2002 microbiological survey Without approval or consultation with anyone in the chain of command, IVINS swabbed a number of locations outside the biocontainment suite on April 15-16, 2002.°% On April 15, 2002, IVINS surveyed the cold-sid pass= u box and the cold-side men's changing room suit In addition, he again swabbed desk area. According to IVINS, he sampled the cold~-side of the pass-thru box because [___urvey yielded a presumptive positive for B.a. on the andle of the pass-thru box inside the[ suite. TVINS swabbed the cold-side men's c ing room because he was concerned about shoes worn inside the suite tracking spores into the changing room. IVINS re-swabbe desk area because he was concerned that since was Still working in and around the B.a. evidence, may have inadvertently contaminated the area. He also wanted to check whether he had done a good job cleaning the desk in December 2001.% On April 16, 2002, IVINS analyzed the results of the previous day's survey and found presumptive positives for B.a. on five of 25 samples. The positives were found on: 1) the molding by the pass-thru box, 2) the men's changing room table, 3) the shelf over the sink in the men's changing room, 4) o he lockers in the men's changing room, and 5) on top of computer hard drive.®* Based on the findings, IVINS continued his independently initiated swabbing survey by obtaining samples from additional areas, to include his office desk area located in room the cold-side[__|pass-thru_box area, and the freezers locatéd in the hallway outside the[__]suite. The areas on which IVINS concentrated were those areas of high traffic, such as a computer keyboard, and those areas of very low traffic, such as the top of a book shelf. Of the 31 samples collected on the second day, IVINS identified approximately 11 which appeared to be presumptive positives. The initial positives from the April 16, 2002, survey were found at the following locations: 1) outside the pass-thru box on the window sill, 2) outside the pass-thru box on the electrical box, 3) IVINS' office on the 0 a