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_ FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID * Continuation of FD-302 of Bruce E. Ivins ,On 09/08/2004 page Typically, IVINS would place a sample in a bag and place it in the autoclave. The last person leaving for the day would usually turn on the autoclave. IVINS reviewed the Reference Material Receipt Record for RMR 1029. He stated that this was not a legal document and was only meant to keep track of the inventory to know when more spores would be needed. IVINS explained that since RMR 1029 were Good Laboratory Practices (GLP) spores, they were not accessible to many people. IVINS reviewed some of the specific entries on the Reference Material Receipt Record_for RMR 1029 and explained the following: (1) Spores used inf _|were for an IM challenge where only thousands of spores were needed. bé Spores for this work were only of RMR 1029 that was b7c then diluted for the study. ( Pores used for Covance were bTP irradiated prior to shipment to Covance’s Denver, ai ee facility for use in spore antiserum production. (3) was [_exoiect, which involved aerosol challenges of approximately 1000 rabbits. [L_|was trying to determine what dose of vaccine would provide protection for 50% of the rabbits challenged. IVINS would take material from RMR 1029 in Suite dilute it, and provide this to for this study. : Lie ecause of (4) Spores used for as undiluted spores. 5) Spores or the Bioport Rabbit Challenge inclnged Po] chat were used to challenge approximately 100 rabbits. (6) Spores for at Battelle were sent for aerosol challenges at Battelle. VI explained that the discrepancies between the volume removed from RMR 1029 May and June 2001 shipments to Battelle | | and respectively) and th 1 actually shipped in May and June 2001 and[o respectively) were due to the fact the IVINS centrifuged the samples and reduced their overall volume prior to shipment to Battelle. (7) Spores provided to on 8/27/01 were undiluted spores. (8) Spores provided to on 10/4/01 were undiluted spores. IVINS explained that RMR 1029 was maintained in two 500 mL flasks in suite B3. When material was needed for a challenge, IVINS would remove the volume of spores needed in the challenge and place this volume in a Gibco serum bottle. This Gibco serum bottle was then transported to building 1412 at USAMRIID, where it was aliquoted for use in the challenge. IVINS stated that the two 500 mL flasks that contained RMR 1029