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, FD-302a (Rev. 10-6-95) 27 9A-WF-222936-USAMRIID Continuation of FD-302 of Bruce E. Ivins ,On 09/08/2004 , Page 6 were never taken over to bui Only the amount needed for a challenge was taken vijainal —I a Gibco serum bottle. The volume of RMR 1029 that was recorded on the Reference Material bé Receipt Record was not based on visual observation but instead b7C was based on back calculati ount taken from the original b7F volume. When asked about a iscrepancy in this record, IVINS explained that evaporation over the years as well as math error would account for this mi ing volume. IVINS stated that RMR 1029 could have lost up to a year and this would not be unusual because it is not stor an an air tight container. He noted that when they aliquoted tubes in the past, they would see an approximate drop in volume over time that they believe is from evaporation. IVINS also stated that he is not sure how. much his record is off from the actual volume because SA took the container and noted there was approximately eft, but did not actually measure the container. Therefore, IVINS is unsure how much of RMR 1029 may be missing. IVINS noted that he shipped some of RMR 1029 to the University of New Mexico in 2001.. IVINS stated that this shipment was not recorded on the Receipt Record and he was not sure why it wasn't recorded. IVINS stated that the information on this shipment would be located on USAMRIID form 11R in the Safety Office. USAMRIID had previously shipped the Ames strain to Dugway in 1992. IVINS does not know what the 1992 shipment was used for at Dugway. IVINS believes that the 1992 shipment to Dugway was spores from either the "1985" or "1989" sample. There is only a small amount of RMR 1029 remaining that is needed for non-human primate (NHP) studies this year. When USAMRIID was getting low on RMR 1029 spores, they contracted Dugway in 2001 to produce additional spores. Dugway put off the production of these spores and just recently sent USAMRIID the last batch of spores they were contracted to produce. The FBI is in possession of the first batch of these spores, called the "2003 Dugway" spores. IVINS is in the process of purifying the last batch of these spores, called the "2004 Dugway" spores. IVINS noted that the 1997 Dugway spores were much nicer than the 2003 Dugway spores. Approximately % of the 2003 shipment could not be used because they were so bad. The only Dugway spores at USAMRIID are the 1997 spores, the 2003 spores, and the 2004 spores. IVINS noted that he called the 1997 Dugway spores the "Dugway Spores". The other two sets of spores were called the