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FD-302a (Rev. 10-6-95) b6 279A-WF-222936-USAMRIID, 279A-BA-C101392 b7C 06/06/2003 3 Continuation ofFD-302 of Page bleach. Swipes of biosafety cabinets following decontamination yielded no growth on culture media, but detectable levels of pxol and pxo2 in some samples. A study was conducted whereby pre-. decontamination sampling was used to identify the 'hottest', or most contaminated, areas in a biosafety cabinet. Detectable levels of DNA were obtained from these areas after decontamination with bleach. The following UsAMRiID personnel may provide further. insights into the impact of various decontamination methods on. subsequent scientific analvses: BRucE IvINs, and Internet searches may yield additional information related to decontamination of biological materials, including diagrams of key mechanisms of inactivation. provided the Agents with a manuscript containing an overview of decontamination technologies and descriptions of the mechanisms of action of numerous decontaminants on microoraanisms. The document, titled DEcONTAMINATION, was co-authored by and SCIENCE APPLICATIONS INTERNATIONAL CORPORATION (SAIC): NATIONAL CANCER INSTITUTE AT FREDERICK, FORT DETRICK, MARYLAND, 21702, telephOne The manuscript is maintained in. a IA envelope (FD- 340) Talso recommended the following textbook as a useful. referenCe: DISINFECTION, STERILIZATION, AND PRESERVATION, 5th edition, Seymour S. Block, Editor, Lippincott Williams & Wilkins, Philadelphia, Pennsylvania, 2ool. Photocopies of the first two pages of the textbook were provided to the Agents and are maintained in a 1A envelope. The Agents were also provided with copies of usAMRIiD's. records of transfers of Bacillus anthracis that occurred from 1997 throuah 1999, as requested in a letter dated June 5, 2003, from. SSA The EA101 forms provided by UsAMRIID are maintained in a 1A envelope.