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FD-302a (Rev. 10-6-95) 279AR-WF-222936 Continuation of FD-302 of a ,On 01/03/06 2 » Page b7C After an aerosol challenge, all material from the challenge was autoclaved. The Ba Ames left in impinger (AGI) was not autoclaved right away. [___]providea the following description of an aerosol challenge: Based_on the number off animals being used in an aerosol challenge,[____]would calculatd the number of spores that[_] would be need for the challenge. BRUCE IVINS would send over the required pyc amount of spores in_an Erlenmey¢r flask or a 50 mL conical tube. b VINS would provide with the Ba Ames from Dugway. IVINS(__]_ pF did not compléte any paperwork documenting the internal Transfer of Ba until approximately mid-2002. IVINS began using an internal transfer sheet and USAMRIID followed very soon after. L___Jprovidea Agents copies of the paperwork documenting the internal transfers of Ba from June 2002 until present. These documents wer ed in an FD-340 and submitted to the 1A section of the file. The aerobiology group began conducting the plate counts approximately 3 years ago. The aerobiology division stores AGI’s in a lock box in the cold storage coonf The lock box has only been in use since approximately June 2 . The material leftover in the AGI’s would usually be approximately 3 orders of magnitude less concentrated than the starting material.