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FD-302a Rev. 10-6-95) <5) mod a a 279R-WF-222936 In April 2004, [ | submitted two sample repository (FBIR). imple labeled "Ames Spores belonged to IVINS. ne contacted IVINS to ask if could submit the sample to the FBIR. [~~] provided a ith an email dated April 6, 2004, in which IVINS responded vf | inquiries regarding the sample. A copy of the email will be submitted to the 1A section of the file. Although the exact history or purpose is unknown, the sample was more likely an AGI tube and not a dilution tube (pre- lenge material). All aerosol challenges occur in building Prior to anthrax_attacks in fall 2001, control and inventory of Ba was very loose. [[__|theorized that samples may have been brought over to[—__]to do a challenge, the challenge may have been cancelled or postponed, and the material may have ended up in the cold room. The only reason that a sample intended for an aeroso ende i ni_down Only were Material IV: ’s aerosol challenges would get plated in aerobiology lab or and then stored in the common use cold room. Not a lot of spores are contained in the AGI’s. It would take hundreds or possibly thousands of AGI’s collected over a long period of time to make up the total amount of material used in the anthrax mailings. In addition material in AGI’s is heavily contaminated and would need to be reisolated to get a pure culture. Plates made from AGI’s would be heavily contaminated as well. recalls the material from the AGI's being heavily contaminated with Staphylococcus and a gram negative bacteria. a not seen a non-anthracis Bacillus species, but it would ifficult to distinguish from Ba. Typically, environmental surveys have indicated Bacillus stearothermophilus present in the basement. This is because this Bacillus species is present on the autoclave indicators tape used on everything that is autoclaved. Ames, and Vollum 1B have been used in aerosol challenges. It is possible that the Sterne strain has also been used. More is looking for a vaccine resistant strain. a fon ave found a vaccine resistant strain from China. No non-anthracis Bacillus strains have been used in aerosol challenges.[7 ould know what strains have been used for challenges. Ba Ames strain from [nes 2 distinct colony types. The Ba material would have to be plated to see the colony types. Ba from the 2001 anthrax letters had the same two distinct colony | Continuation of FD-302 of :On_ 01/03/06 _,Page_ 4