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ALL NFORM&TION CONTAINED HERE UNCLASSIFIED DATE 12-10-2006 60324 UC BAW/RS/LSC SPORE PREPARATION FORM be STUDY #: GL/~ Jo4/-3-Le DATE: /0//6/q" INVESTIGATOR: Brace 775, < Ry long SPORES USED: B. anthracis Ames strain, reference material: » in 1% phenol APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3 2.X/@%/217/y / DESIRED CONCENTRATION OF SPORES FOR AEROSOL: [ DILUTION FACTOR: 1 ml of spores per ml of aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED: [] DED FOR AEROSOL: [ AMOUNT OF SPORES ADDED TO BOTTLE: AMOUNT OF WATER ADDED TO BOTTLE: SPORES DELIVERED TO BE AEROSOLIZED AT: 3/30 am 1016 9 > PLATE COUNTS from DELUTION): PLATE 1 PLATE2 PLATE3 PLATE4 PLATES 32 37 4/ 35 aad AVERAGE= 33,2. CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: JVone bé b7c SIGNATURE OR INITIALS: DATE: /0 //@/¢ -D DATA VERIFIED BY: DATE: /0//9 1/9 2 > AwVT 0 0 Avi 1