◆ SpookStack

ALL NFORMATION CONTAINED | * HERE UNCLASSIFIED . vo rmenenvecon ATE [2-10-2008 BY 60324 UC BAW/RS/LSt SPORE PREPARATION FORM STUDY #: D99-02 DATE: 15 OCT 99 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames, RMR 1029, in 1% phenol APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 2.5 X 10"ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL ___| DILUTION FACTOR: 1 mi of spores pe{—_or aerosol suspension NUMBER OF ANIMALS TO BE cHaLtencepl_| AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR asrosot{ | AMOUNT OF SPORES ADDED TO BOTTLE; _pucrotters AMOUNT OF WATER ADDED TO BOTTLE: | Imi SPORES DELIVERED TO BE AEROSOLIZED AT: 0730 PLATE COUNTS (FROM _ DILUTION): PLATE1 PLATE2 PLATE3 PLATE4 PLATES “0 a! He 2¢ 32 AVERAGE= 2.51, CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR INITIALS: bA DATE: /o//$ fag DATA VERIFIED BY: | | DATE: fu C4 47