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¥ FD-302a (Rev. 10-6-95) @ 279A-WF-222936-USAMRIID [-Jsaia that the same AGIs have been used for at least the past ten (10) years. Recently the AGIs have been modified to use a screw top instead of a push on-top. The material that was collected in the AGI would be maintained for several days or longer following a challenge. Small amounts of the antifoam were aliquoted into tubes. In previous years, larger tubes were used which caused excess waste. noted that Anthrax spores are very resilient. Lis unaware of any antifoam stock ever reported missing. [__]eaia that[___]would assume thatL—=dYd knew the process of using antifoam_as part of the aerosol challenges. jin the past olive oil was used for aerosol challenges. The olive oil was used for challenges involving toxin proteins. Oliv i years. as not seen a bottle of olive oil at USAMRIID since the early 1990's. CL Jstatea that there is no difference in the post challenge assays if olive oil or antifoam are used during the aerosol challenge.