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FD-302a (Rev. 10-6-95) @ 279A-WF-222936-USAMRIID conininorrosnt [Td ,0n.04/07/2004 Page __4 said that was not around for any of the aerosol challenges related t studies. never saw the post challenge plates after they had been placed into the autoclave bags for disposal. : | there are unofficial and also possibl official procedures for anthrax aerosol challenges (oe IVINS would have a copy of the_procedure, as well as a protocol for anthrax aerosol preparation. Po] there are general Standard Operating Procedures (SOP) for equipment usage and maintenance. stated that the systems used in the aerosol challenge process have been updated_with newer, automated, computer based systems. As an example. ___—_| the flow meters are now electronic instead of manual. Some of the modifications to the units over the past several years have been based on system maintenance, however, other modifications have involved complete component replacements. Upgrades made to the equipment were not noted in a log. oe maintenance logs were maintained for filters, tubing, and glove replacements. The maintenance logs were not kept with the intention of historical documentation. The hood lines present in the laboratory now are the same ones present since the early 1990's. On the National Institute of Allergy and Infectious Diseases (NIAID) website, interviewing agents should be able to locate a presentation given at an aerosol workshop. The presentation was given in December of 2003. The presentation should have photographs of the equipment used in the aerosol challenges. bé Cer sear ep anal (Ba) Ames challenges are j7c still conducted at USAMRIID. one non-human primate is going to be exposed to Ba Ames around May 1, 2004. The PI for the study is IVINS will provide the Ba Ames for the challenge. there will be an aerosol challenge of Ba Ame involving mice at the end of April 2004. The PI for the study is Both of the upcoming challenges will take place in room of uilding the aerosol technicians listed on the DIC Aerosol Challenge Log sheets were the people actually running the challenge. [it rabbits were utilized in the challenge, individual would need to be involved to hold the animal. there were always people around observing the challenges,