◆ SpookStack

' repeat tlie work of Santesson ahd determine the best means of Extracting the active ^onstltuent(s) . ^ Finally a 100 g. sample of the seed/crushed to pass -a. *5 mesh sieve, was . ^ hk^acted in a sohxlet^ith" petroleum ether to remove oils. v'-The" de-oiled seed was maXoerated with distilled water and the mixture centrifuged to separate the marc. This marc apparently .was "devoid' of“alkaloid - or- glycosides extractable .with various polar and non-porarTorganic solvents. The aqueous extract was" freed from most protein material by the use of trichloroacetic acid. The deproteinated extract was neutralized by means of ion exchange resins. The ' effluent from the resin column was concentrat ed in vacuo / resulting in the precipitublon^f j more gummy material . : The. supernatant was-clear, viscous, dark green and intensely bitter It contained no free reducing 3ugars , Tests with alkaioidal reagents were inconclusive. Attempts to extract - alkaioidal material from this solution using various solvents were unsuccessful. Imulsions interfered with the extraction in both neutral and basic solution. The aqueous solution was stirred with methanol, a procedure which removed most of the polysaccharides, which were presept in large quantities and decreased the viscosity of the aqueous solution. The resulting aqueous -alcoholic solution was concentrated to remove methanol. It gave positive tests with all common alkaioidal reagents and negative tests for reducing sugars. WARNING NOTICE v " ' '* • : -'V-