◆ SpookStack

>f“ ; ** t . > - • WW** 1 L»S^BtBfeS3aPa’~ ' -„•>• >. I,. - A *2 -; - , <-?-*> , -, 4'".J.< ’ - •/' si-- ', 1 ' . ®;V: ’< <v i?: t-v r'5-5^- f . ,,. - . -~a. minute quantity of crystalline material. formed In ‘V 1 . /;•' . < v: : ' ,'W*' --- •/' , . - r -.• - ;i" ;0;SStM3 aqueous' solution after a day. This material has ^;S The remainder of the solution '. Tt'VrV^ water bath, in a nitrogen atmosphere, ; ; ^ material, ^he taken up in water and more polysaccharides r -: preeipitated with the addition of methanol. Repeating • •5;\;;v.v: ..;>• ';. ; '•• this •'procedure eventually-removedfall of: the poly- - Lv ” saccharides From the supernatant, after removal of polysaccharides, crystalline material was obtained. • \-;-r :£ ^;A--TesW : f>n this solution indi cated the pre sence of v ;>--.yy>V5-'4ihaioid3 and the absence; of reducing sugars . This eolutlon^aiso' was intensely bitter, a crude indication > ^ of the presence ^f the active prlnciple(3) of BFiule tt , •••.•; No' : test''aniiaals.'.were available at the time for physiology y •^•;v^i'ic^'- % tes'ts various fractions. This solution was : : ; :{ hydrolyzed with 2N H2SO4. A voluminous amount of pre- T ; -\ .: ; c ipitate resulted. The clear supernatant yielded y "excellent tests for alkaloids and reducing sugars. • . paper chromatography was employed to confirm the presence of reducing sugars. The polysaccharides were hydrolyzed and the resulting solution, after neutral- ization, was subjected to paper chromatography. The results are 'incomplete j however, glucose, mannose and xylose appear to be the predominate monosaccharides present, WARNING NOTICE *