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. . . ALL INFORMATION CONTAINED t e HER 3 UNCLASSIFIED DATE [2-10-2008 BY 60324 UC BAllyRS/LSC | SPORE PREPARATION FORM STUDY #: B00-03 (part 2: 1 dose efficacy with 25 pg PA; 2 preparations) vi 8 sux. 00 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10" /ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL[ | DILUTION FACTOR: 1 ml of spores per Lor aerosol suspension NUMBER OF ANIMALS TO BE cuattencen{ | AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR arRosoL[ | AMOUNT OF SPORES ADDED TOBOTTLE:[_| AMOUNT OF WATER ADDED TO iq | SPORES DELIVERED TO BE AEROSOLIZED AT: morning 0 ch July 00 PLATE COUNTS (FROM|__ bILUTION): PLATE1 PLATE2 PLATE3 PLATE4 PLATES QE 3{ 38 a4 B AVERAGE= 30.Q. CONCENT A IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR INITIALS: &, - DATE: 62S Je/o 0 be , b7c DATA VERIFIED BY: DATE: 5/ fil 00