◆ SpookStack

HERE, UNCLASSIFIED DATE [2-10-2008 BY 60324 UC BAW/RS/LSC ® ALL INFORMATION CONTAINED SPORE PREPARATION FORM STUDY #: B00-03 (part 2: 1 dose efficacy with 25 yg PA; 2 preparations) DATE: HFUE00 SPORE PREPARER: Ivins 52 ¥1 July 00 bie SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10” /ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL: DILUTION FACTOR: 1 ml of spores per [j= of aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED: AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR AEROSOL: [| AMOUNT OF SPORES ADDED TO sorte, | AMOUNT OF WATER ADDED TO sorne{ | oj M1 daly 00 SPORES DELIVERED TO BE AEROSOLIZED AT: merning-of 11 July-00 PLATE COUNTS FROM _ DILUTION): PLATE PLATE2 PLATE3 PLATE4 PLATES 22 ab 30 3Y 37 AVERAGE= 30 CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR INITIALS: - 2 DATE: ]63,,/00 DATA VERIFIED “| DATE: 4/ ‘nl oo