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@ ALL FSI INFORMATION CONTAINED yp HE] '$ UNCLASSIFIED DATS 10-2008 BY 50324 UC Bau/RS/LSC SPORE PREPARATION FORM STUDY #: B00-03 (part 1: 1-dose efficacy test with 2 PA preparations) DATE: 7 April 00 SPORE PREPARER: Ivins raed 11 5 SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION:[_____| DESIRED CONCENTRATION OF SPORES FOR AzROsOLL DILUTION FACTOR: 1 ml of spores per [ |m of aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED] | AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR AEROSOL: [| AMOUNT OF SPORES ADDED TO a AMOUNT OF WATER ADDED TO BOTTLE: [| SPORES DELIVERED TO BE AEROSOLIZED AT: morning of 7 April 00 PLATE COUNTS (ROM, __ pILuTION): PLATE1 PLATE2 PLATE 3 PLATE 4 PLATE 5 2: 36 30 47 3e AVERAGE= 34 7 CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: SAIKIO | GHG KO? 2 3.46 KIO pf to"? OTHER REMARKS: bé . b7c SIGNATURE OR mms] | pate: 4, [2 o/0d DATA VERIFIED BY: £752 DATE: bf Ca