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a ALL INFORMATION CONTAINED HER! ‘SS UNCLASSIFIED DATE 12-10-2008 BY 60324 UC BAU/RS/LSC SPORE PREPARATION FORM STUDY #: BO0-03 (part 1: 1-dose efficacy test with 2 PA preparations) DATE: 10 April 00 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10%/ml DESIRED CONCENTRATION OF SPORES FOR arrosoLr{ i DILUTION FACTOR: 1 ml of spores per [ok aerosol suspension NUMBER OF ANIMALS TO BE cuatxencso] —_| AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR agRosoL{ | ; AMOUNT OF SPORES ADDED TOBOTILE:[___| AMOUNT OF WATER ADDED TO BoTTLE:[|___| SPORES DELIVERED TO BE AEROSOLIZED AT: morning of 10 April 00 PLATE COUNTS FROM { _|DILUTION): PLATE1 PLATE2 PLATE3 PLATE4 PLATES fo) a 4O SY $4 AVERAGE= 41,4 CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: SIGNATURE OR INITIALS: DATA VERIFIED BY: