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r ) ALL INFORMATION CONTAINED HEI DATE 12: UNCLASSIFIED -Z2008 BY 60324 UC BAW/RA/LSC SPORE PREPARATION FORM STUDY #: B00-03 (part 1: 1-dose efficacy test with 2 PA preparations) DATE: 5 April 00 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames strain (RMR 1029) APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10"/ml DESIRED CONCENTRATION OF SPORES FOR agrosorf | DILUTION ractor{__lor spores per [oh aerosol suspension be NUMBER OF ANIMALS TO BE CHALLENGED[__| AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR arroso| —_| AMOUNT OF SPORES ADDED TOBOTILE, _| AMOUNT OF WATER ADDED TOBOTTLE[ _| SPORES DELIVERED TO BE AEROSOLIZED AT: morning of 5 April 00 PLATE COUNTS (FROM|___ PILUTION): PLATE1 PLATE2 PLATE3 PLATE4 PLATES 2] 19 |B BR BS AVERAGE = if CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: bé SIGNATURE OR wmas| | DATE: [2s /v v b7c DATA VERIFIED BY: pr DATE: SP /éo